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DETECTION OF VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI AND CHARACTERIZATION OF SUB-VARIANT OF stx GENES ISOLATED FROM THE ZANGO ABATTOIR AND SURROUNDING


LAWAN, M.K.1*, BELLO, M.1, KABIR, J.1 AND MORABITO, S.2

1Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria 2European Reference Laboratory for Verocytotoxin Escherichia coli, Rome, Italy

Abstract:

Escherichia coli O157:H7 and other non-O157 verotoxin-producing E. coli serotypes also known as Shiga-like producing E. coli (STEC) have been associated with human diseases as foodborne pathogens responsible for outbreaks and sporadic cases of diarrhoea, haemolytic uraemic syndrome (HUS) and haemorrhagic colitis. It is essential to generate further information on the occurrence of different serotypes of STEC, subvariants of stx1 and stx2 genes circulating in the abattoir environment. Cattle faeces, abattoir effluent, water samples from stream receiving the abattoir effluent and vegetables samples irrigated with water from the stream were taken from the abattoir environment. E. coli was phenotypically isolated from these samples using EMB agar and biochemically characterised. Biochemically characterised E. coli were subjected to conventional PCR and microtitre agglutination test to identify stx genes, subtypes of stx genes variants and also to serotype the isolated STEC isolates. The results of the characterisation showed the presence of both stx1 and stx2 genes in the three isolates of STEC. However O-serogroups O91 and O8 were identified from STEC isolates. In conclusion this study demonstrates the isolation of three STECs from cattle faeces and cabbage samples harbouring stx1a and stx2 (stx2a, stx2b, stx2d) genes which are predisposing factors towards development of HUS in human infection.

Key words: Verotocytotoxin,E. coli, abattoir, environment, stx genes, public health

*Correspondence: kaboskylawal@yahoo.com

How to cite: Lawan et al. (2017); Verocytotoxin-producing Escherichia coli in abattoir; Nigerian Journal of Scientific Research, 16(4): 2017; July - August; njsr.abu.edu.ng

 

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